Dr. Ramani’s research focuses on the mechanisms that control how genes are turned on or off in our cells. With his expertise in genomic technology development, single-cell sequencing, gene regulation, chromatin structure, dosage compensation, and RNA biology, a key research interest is the development of new high-throughput molecular techniques that make it possible to monitor gene activity at the level of single cells and single molecules. With these techniques, Ramani and his team study how changes in genome organization affect gene activity, or how the same gene may produce different RNA molecules under different conditions. In turn, the team hopes to parlay this information into a better understanding of the molecular steps that lead to disease or maintain the pluripotency of stem cells.